An Age of Drug Discovery for Diseases of the Elderly: The Role of Senolytics
Synthesis and biological evaluation of new quinoline and anthranilic acid
In the world of pharmaceutical development, the significance of chirality cannot be overstated. With many drugs existing as racemic mixtures, the ability to separate enantiomers efficiently is crucial. Preparative supercritical fluid chromatography (SFC), is a relatively new technique that has emerged as a powerful tool for chiral separations. This post explores the advancements and applications of preparative SFC and highlights its advantages over traditional methods.
Proin sodales pulvinar sic tempor. Sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Nam fermentum, nulla luctus pharetra vulputate, felis tellus mollis orci, sed rhoncus pronin sapien nunc accuan eget.
Sociis natoque penatibus et magnis dis parturient montes, nascetur ridiculus mus. Nam fermentum, nulla luctus pharetra vulputate, felis tellus mollis orci, sed rhoncus pronin sapien nunc accuan eget.
Understanding Chirality and Its Importance
Chirality refers to the property of a molecule having non-superimposable mirror images, like the left and right hands. In pharmaceuticals, different enantiomers of a chiral drug can exhibit vastly different biological activities. For instance, the well-known Thalidomide incident serves as a reminder of the consequences of using racemic mixtures without proper separation. Thus, the quest for efficient enantiomer separation has been a key focus in drug discovery and development for over two centuries.
In the world of pharmaceutical development, the significance of chirality cannot be overstated. With many drugs existing as racemic mixtures, the ability to separate enantiomers efficiently is crucial. Preparative supercritical fluid chromatography (SFC), is a relatively new technique that has emerged as a powerful tool for chiral separations. This post explores the advancements and applications of preparative SFC and highlights its advantages over traditional methods.
Graphical representation of SFC Prep 150 AP prep systemAdvantages of Preparative SFC
- Environmental Benefits: SFC utilizes supercritical carbon dioxide, a non-toxic and renewable solvent that can even be recycled in the process. This aligns with the principles of green chemistry, making SFC a sustainable choice for large-scale separations where toxic solvents like hexane are used.
- Enhanced Efficiency: The unique properties of supercritical CO2, such as lower viscosity, UV-transparency in the detection range, and high diffusivity, facilitate faster separations and higher product concentrations. This leads to improved productivity, which is vital for pharmaceutical manufacturing.
- Versatility in Applications: Preparative SFC has found applications across various fields, including food science, natural products, and, most notably, pharmaceuticals. Its ability to separate chiral compounds makes it an indispensable tool in drug development.
The Evolution of Supercritical Fluid Chromatography
The evolution of instrumentation has played a crucial role in the success of preparative SFC. The first SFC/MS instrument was introduced in 1978. Today, Mass Spectrometry (MS) detection is gaining popularity due to the valuable insights it offers and the advancement of affordable MS detectors. Modern and more automated devices have improved the resolution and efficiency of separations, allowing for the handling of larger sample volumes. New generation systems like our Waters system SFC Prep 150 AP has opened new avenues for SFC.
These systems offer precise control over pressure, temperature, and flow rate. State-of-the-art components, including enhancements to the pump head, back pressure regulator, and UV flow cell, have significantly reduced UV noise while improving detection in preparative separation while components as gas-liquid separator (GLS) have improved the recovery efficiency while techniques such as stacked injection systems have further enhanced productivity.
Drug discovery
FAQ
Selvita leverages advanced screening technologies and analytical tools for faster, more precise, and scalable compound identification tailored to research needs.
Selvita leverages advanced screening technologies and analytical tools for faster, more precise, and scalable compound identification tailored to research needs.
Selvita leverages advanced screening technologies and analytical tools for faster, more precise, and scalable compound identification tailored to research needs.
Selvita leverages advanced screening technologies and analytical tools for faster, more precise, and scalable compound identification tailored to research needs.
Selvita leverages advanced screening technologies and analytical tools for faster, more precise, and scalable compound identification tailored to research needs.
References:
- L.T. Taylor, Supercritical fluid chromatography for the 21st century, J. Supercrit. Fluids 47 (2009) 566–573.
- L.G. Randall, A.L. Wahrhaftig, Dense gas chromatography/mass spectrometer interface, Anal. Chem. 50 (1978) 1703–1705
- Speybrouck, D., & Lipka, E. (2016). Preparative supercritical fluid chromatography: A powerful tool for chiral separations. Journal of Chromatography A, 1467, 33–55.
